We would like to join a project aimed at Survey of Tospovirus species on crops in Egypt governorates. Different Egypt governorates, plants showing possible virus-caused symptoms (including different crops will collect and will test by antibodies and used for the field survey. For confirmation of the serological analysis, RT-PCR will carry. Tospovirus, which is a genus in the family Bunaviridae, is one of the most important plant viruses causing significant economic losses on a wide range of crops. Tomato spotted wilt virus (TSWV) is the type member of this genus. Fourteen distinct species are currently placed in this genus on the basis of their nucleotide sequence identities. Since Tospovirus is transmitted by insect vectors (Thrips) and the disease spreads in a short period, rapid diagnosis of the disease is indispensable. Biological properties, such as electron microscopy observations or host ranges, are used to diagnose virus diseases, however, serological and molecular methods are currently used for rapid and precise diagnosis. There are many Tospovirus species. Among them, Chrysanthemum stem necrosis virus , which causes great losses to melon and cucumber production, Groundnut ringspot virus infect peanut crop, Impatiens necrotic spot virus , Tomato spotted wilt virus on Vegetables (tomato& broad bean& lettuce& pea& pepper& potato& celery& eggplant& celery& spinach). Tomato chlorotic spot virus, , Iris yellow spot virus on ornamentals plants,Tomato yellow fruit ring virus infected On tomato ,Groundnut bud necrosis virus ,Calla lily chlorotic spot virus ,Melon yellow spot virus, Watermelon silver mottle virus, Peanut chlorotic fan-spot virus, Capsicum chlorosis virus ,and Polygonum ring spot virus .
life History thrip: Tospoviruses are transmitted by vegetative propagation of infected plant material and by the feeding activity of certain species of thrips. In greenhouses, the most important vector is considered to be the western flower thrips (WFT), Frankliniella occidentalis. The viruses is associated with thrips in a persistent manner.
Larvae become viruliferous after feeding about 30 minutes on an infected plant. After a latent period lasting 3 to 18 days, the thrips can infect new plants after feeding for 5 to 10 minutes.
During feeding, saliva is injected into the plant cell and the contents withdrawn. When Tospoviruses are present in the saliva, these are transmitted to the plant during the feeding process in as little as 15-30 minutes. Only the two larval instars of WFT can acquire the virus. Adult WFT do not acquire the virus from tospovirus-infected plants because tospoviruses pass right through the gut and do not enter the salivary fluid. Once thrips acquire Tospoviruses multiplies within the salivary glands and other tissues of the thrips and is later transmitted to new plants by the feeding of the infected thrips after it has reached the adult stage. Adults however, do not transmit the virus to their young and there is no transmission from adult to egg. Overlapping generations of thrips within a greenhouse may result in continuous virus transmission. In greenhouses the viruses can be perpetuated through successive crops and weeds resulting in losses year-round.
Why is it important?
range of crops damaged, increasing tosposvirus number and global
distribution.Tansmission by thrips vector and vegative propagation, lack of seed transmission.
Critical features of epidemiology and importance of IDM for control in
seed production Limited host reistance available for breeding
Climate change will increase distribution, emergence, severity, epidemic unpredictabillity, losses;
Why you are motivated by this research?
Because this group of viral task groups, which cause significant losses for a wide range of plant families.
I am looking forward a prima project as a partner for preventing emergence of plant pests (Thrips) and reduce Tosposvirus .
DESCRIPTION OF YOUR ORGANISATION:
Plant Pathology Research Institute (PPathRI) strives to meet the demands of government agricultural policies, particularly those of national concern, while at the same time fulfilling industry needs. The institute conducts plant disease surveys, carries out research for the detection of causal organisms, quantifies crop loss assessment and develops management alternatives for control. Among its other activities, the institute supports the country's quarantine service, including examinations of imported and exported plant materials.
The Partnership for Research and Innovation in the Mediterranean Area will devise new R&I approaches to improve water availability and sustainable agriculture production in a region heavily distressed by climate change, urbanisation and population growth